ОКСИДАТИВНЫЙ СТАТУС ЭРИТРОЦИТОВ ПОСЛЕ МАКСИМАЛЬНОГО АЭРОБНОГО ТЕСТА У БОРЦОВ
Ключевые слова:
эритроциты, максимальный аэробный тест, оксидативный статус, борцы
Аннотация
Цель. Данное исследование проводилось с целью выявления изменений в оксидативном статусе эритроцитов у борцов после выполнения максимального аэробного теста. В рамках исследования проводилась регистрация уровня пероксидации липидов, общего глутатиона, активности каталазы, супероксиддисмутазы и глутатионпероксидазы. Материалы и методы. 12 здоровых борцов приняли участие в максимальном аэробном тесте на беговой дорожке. Перед тестированием и сразу после выполнения упражнения у участников были взяты образцы венозной крови. Из плазмы крови были выделены эритроциты, в которых методом спектрофотометрии были исследованы пероксидация липидов, общий глутатион и активность ферментов. Плазма была также использована для определения концентрации гемоглобина как показателя гемолиза. Результаты. Выполнение максимального аэробного теста спровоцировало значительное повышения уровня гемоглобина в плазме крови и снижение пероксидации липидов, однако никак не повлияло на уровень общего глутатиона в эритроцитах. Что касается антиоксидантных ферментов, то результаты анализа показали увеличение активности глутатионпероксидазы, при этом активность каталазы и супероксиддисмутазы осталась на том же уровне. Заключение. Можно прийти к выводу, что у активных спортсменов преобладают эритроциты, которые более устойчивы к оксидативному стрессу вследствие ускоренного гемолиза, спровоцированного физическими упражнениями, которые приводят к удалению старых и оксидативно модифицированных клеток.
Литература
1. Aebi H. Catalase in Vitro. Methods in Enzymology, 1984, vol. 105, pp. 121–126. DOI: 10.1016/S0076-6879(84)05016-3
2. Alexandrova A., Eroglu Y., Petrov L., Makaveev R., Georgieva A., Tzvetanova E. Blood Plasma Oxidative Stress Parameters after Maximal Oxygen Consumption Test in Wrestlers. International Journal of Sport Studies, 2016, vol. 6, pp. 359–366.
3. Beauchamp C., Fridovich I. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels. Anal Biochem, 1971, vol. 44, pp. 276–287. DOI: 10.1016/0003-2697(71)90370-8
4. Bogdanska J.J., Korneti P., Todorova B. Erythrocyte Superoxide Dismutase, Glutathione Peroxidase and Catalase Activities in Healthy Male Subjects in Republic of Macedonia. Bratislavské Lekárske Listy, 2003, vol. 104, pp. 108–114.
5. Dane S., Taysi S., Gul M., Akcay F., Gunal A. Acute Exercise Induced Oxidative Stress is Prevented in Erythrocytes of Male Long Distance Athletes. Biology of Sport, 2008, vol. 25, pp. 115–124.
6. Djordjevic D., Cubrilo D., Macura M., Barudzic N., Djuric D., Jakovljevic V. The Influence of Training Status on Oxidative Stress in Young Male Handball Players. Molecular and Cellular Biochemistry, 2011, vol. 351, pp. 251–259. DOI: 10.1007/s11010-011-0732-6
7. Fisher-Wellman K., Bloomer R.J. Acute Exercise and Oxidative Stress: a 30 Year History. Dynamic Medicine: DM, 2009, vol. 8, p. 1.
8. Gilbert H.S., Stump D.D., Roth E.F. A Method to Correct for Errors Caused by Generation of Interfering Compounds During Erythrocyte Lipid Peroxidation. Analytical Biochemistry, 1984, vol. 137, pp. 282–286. DOI: 10.1016/0003-2697(84)90086-1
9. Günzler W.A., Vergin H., Müller I., Flohé L. Glutathione Peroxidase VI: the Reaction of Glutahione Peroxidase with Various Hydroperoxides. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie, 1972, vol. 353, pp. 1001–1004.
10. Hanzawa K., Kai M., Hiraga A., Watanabe S. Fragility of Red Cells During Exercise is Affected by Blood pH and Temperature. Equine Veterinary Journal, 1999, suppl. 610–1.
11. Hubner-Wozniak E., Panczenko-Kresowska B., Starczewska-Czapowska J. The Effect of Graded Exercise on Changes in the Activity of Antioxidant Enzymes in Blood of Wrestlers. BiolSport, 1993, vol. 10, pp. 21–22.
12. Iuchi Y. (2012) Anemia Caused by Oxidative Stress. In: Silverberg DS (ed) Anemia. 2012. 480 p. DOI: 10.5772/31404
13. Kaminsky L.A., American College of Sports Medicine., American College of Sports Medicine. ACSM’s Health-Related Physical Fitness Assessment Manual. Wolters Kluwer Health/Lippincott Williams & Wilkins. 2014.
14. Rifkind J.M., Nagababu E., Ramasa- my S., Ravi L.B. Hemoglobin Redox Reactions and Oxidative Stress. Redox Report: Communications in Free Radical Research, 2003, vol. 8, pp. 234–237. DOI: 10.1179/135100003225002817
15. Selby G.B., Eichner E.R. Endurance Swimming, Intravascular Hemolysis, Anemia, and Iron Depletion. New Perspective on Athlete’s Anemia. The American Journal of Medicine, 1986, vol. 81, pp. 791–794. DOI: 10.1016/00029343(86)90347-5
16. Smith J.A., Kolbuch-Braddon M., Gillam I., Telford R.D., Weidemann M.J. Changes in the Susceptibility of Red Blood Cells to Oxidative and Osmotic Stress Following Submaximal Exercise. European Journal of Applied Physiology and Occupational Physiology, 1995, vol. 70, pp. 427–436. DOI: 10.1007/BF00618494
17. Spasic M.B. Antioxidative Defence in Mammals – A Review. Jugoslov Med Biohem, 1993, vol. 12, pp. 1–9.
18. Tauler P., Aguiló A., Guix F., Jiménez F., Villa G., Tur J., Córdova A., Pons A. PreExercise Antioxidant Enzyme Activities Determine the Antioxidant Enzyme Erythrocyte Response to Exercise. J Sports Science, 2005, vol. 23, pp. 5–13. DOI: 10.1080/02640410410001716724
19. Tietze F. Enzymic Method for Quantitative Determination of Nanogram Amounts of Total and Oxidized Glutathione: Applications to Mammalian Blood and Other Tissues. Analytical Biochemistry, 1969, vol. 27, pp. 502–522. DOI: 10.1016/0003-2697(69)90064-5
20. Winterbourn C.C. Haemoglobin Oxidation and Free Radical Production in the Red Cell. Biomedica Biochimica Acta, 1983, vol. 42, pp. 134–138.
21. WMA. WMA Declaration of Helsinki – Ethical Principles for Medical Research Involving Human Subjects. 2013. Available at: http://www.wma.net/en/30publications/10policie s/b3/. Accessed 27.07.2016.
22. Zavodnik L.B., Zavodnik I.B., Niekurzak A., Szosland K., Bryszewska M. Activation of Red Blood Cell Glutathione Peroxidase and Morphological Transformation of Erythrocytes Under the Action of Tert-Butyl Hydroperoxide. Biochemistry and Molecular Biology International, 1998, vol. 44, pp. 577–588. DOI: 10.1080/15216549800201612
2. Alexandrova A., Eroglu Y., Petrov L., Makaveev R., Georgieva A., Tzvetanova E. Blood Plasma Oxidative Stress Parameters after Maximal Oxygen Consumption Test in Wrestlers. International Journal of Sport Studies, 2016, vol. 6, pp. 359–366.
3. Beauchamp C., Fridovich I. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels. Anal Biochem, 1971, vol. 44, pp. 276–287. DOI: 10.1016/0003-2697(71)90370-8
4. Bogdanska J.J., Korneti P., Todorova B. Erythrocyte Superoxide Dismutase, Glutathione Peroxidase and Catalase Activities in Healthy Male Subjects in Republic of Macedonia. Bratislavské Lekárske Listy, 2003, vol. 104, pp. 108–114.
5. Dane S., Taysi S., Gul M., Akcay F., Gunal A. Acute Exercise Induced Oxidative Stress is Prevented in Erythrocytes of Male Long Distance Athletes. Biology of Sport, 2008, vol. 25, pp. 115–124.
6. Djordjevic D., Cubrilo D., Macura M., Barudzic N., Djuric D., Jakovljevic V. The Influence of Training Status on Oxidative Stress in Young Male Handball Players. Molecular and Cellular Biochemistry, 2011, vol. 351, pp. 251–259. DOI: 10.1007/s11010-011-0732-6
7. Fisher-Wellman K., Bloomer R.J. Acute Exercise and Oxidative Stress: a 30 Year History. Dynamic Medicine: DM, 2009, vol. 8, p. 1.
8. Gilbert H.S., Stump D.D., Roth E.F. A Method to Correct for Errors Caused by Generation of Interfering Compounds During Erythrocyte Lipid Peroxidation. Analytical Biochemistry, 1984, vol. 137, pp. 282–286. DOI: 10.1016/0003-2697(84)90086-1
9. Günzler W.A., Vergin H., Müller I., Flohé L. Glutathione Peroxidase VI: the Reaction of Glutahione Peroxidase with Various Hydroperoxides. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie, 1972, vol. 353, pp. 1001–1004.
10. Hanzawa K., Kai M., Hiraga A., Watanabe S. Fragility of Red Cells During Exercise is Affected by Blood pH and Temperature. Equine Veterinary Journal, 1999, suppl. 610–1.
11. Hubner-Wozniak E., Panczenko-Kresowska B., Starczewska-Czapowska J. The Effect of Graded Exercise on Changes in the Activity of Antioxidant Enzymes in Blood of Wrestlers. BiolSport, 1993, vol. 10, pp. 21–22.
12. Iuchi Y. (2012) Anemia Caused by Oxidative Stress. In: Silverberg DS (ed) Anemia. 2012. 480 p. DOI: 10.5772/31404
13. Kaminsky L.A., American College of Sports Medicine., American College of Sports Medicine. ACSM’s Health-Related Physical Fitness Assessment Manual. Wolters Kluwer Health/Lippincott Williams & Wilkins. 2014.
14. Rifkind J.M., Nagababu E., Ramasa- my S., Ravi L.B. Hemoglobin Redox Reactions and Oxidative Stress. Redox Report: Communications in Free Radical Research, 2003, vol. 8, pp. 234–237. DOI: 10.1179/135100003225002817
15. Selby G.B., Eichner E.R. Endurance Swimming, Intravascular Hemolysis, Anemia, and Iron Depletion. New Perspective on Athlete’s Anemia. The American Journal of Medicine, 1986, vol. 81, pp. 791–794. DOI: 10.1016/00029343(86)90347-5
16. Smith J.A., Kolbuch-Braddon M., Gillam I., Telford R.D., Weidemann M.J. Changes in the Susceptibility of Red Blood Cells to Oxidative and Osmotic Stress Following Submaximal Exercise. European Journal of Applied Physiology and Occupational Physiology, 1995, vol. 70, pp. 427–436. DOI: 10.1007/BF00618494
17. Spasic M.B. Antioxidative Defence in Mammals – A Review. Jugoslov Med Biohem, 1993, vol. 12, pp. 1–9.
18. Tauler P., Aguiló A., Guix F., Jiménez F., Villa G., Tur J., Córdova A., Pons A. PreExercise Antioxidant Enzyme Activities Determine the Antioxidant Enzyme Erythrocyte Response to Exercise. J Sports Science, 2005, vol. 23, pp. 5–13. DOI: 10.1080/02640410410001716724
19. Tietze F. Enzymic Method for Quantitative Determination of Nanogram Amounts of Total and Oxidized Glutathione: Applications to Mammalian Blood and Other Tissues. Analytical Biochemistry, 1969, vol. 27, pp. 502–522. DOI: 10.1016/0003-2697(69)90064-5
20. Winterbourn C.C. Haemoglobin Oxidation and Free Radical Production in the Red Cell. Biomedica Biochimica Acta, 1983, vol. 42, pp. 134–138.
21. WMA. WMA Declaration of Helsinki – Ethical Principles for Medical Research Involving Human Subjects. 2013. Available at: http://www.wma.net/en/30publications/10policie s/b3/. Accessed 27.07.2016.
22. Zavodnik L.B., Zavodnik I.B., Niekurzak A., Szosland K., Bryszewska M. Activation of Red Blood Cell Glutathione Peroxidase and Morphological Transformation of Erythrocytes Under the Action of Tert-Butyl Hydroperoxide. Biochemistry and Molecular Biology International, 1998, vol. 44, pp. 577–588. DOI: 10.1080/15216549800201612
2. Alexandrova A., Eroglu Y., Petrov L., Makaveev R., Georgieva A., Tzvetanova E. Blood Plasma Oxidative Stress Parameters after Maximal Oxygen Consumption Test in Wrestlers. International Journal of Sport Studies, 2016, vol. 6, pp. 359–366.
3. Beauchamp C., Fridovich I. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels. Anal Biochem, 1971, vol. 44, pp. 276–287. DOI: 10.1016/0003-2697(71)90370-8
4. Bogdanska J.J., Korneti P., Todorova B. Erythrocyte Superoxide Dismutase, Glutathione Peroxidase and Catalase Activities in Healthy Male Subjects in Republic of Macedonia. Bratislavské Lekárske Listy, 2003, vol. 104, pp. 108–114.
5. Dane S., Taysi S., Gul M., Akcay F., Gunal A. Acute Exercise Induced Oxidative Stress is Prevented in Erythrocytes of Male Long Distance Athletes. Biology of Sport, 2008, vol. 25, pp. 115–124.
6. Djordjevic D., Cubrilo D., Macura M., Barudzic N., Djuric D., Jakovljevic V. The Influence of Training Status on Oxidative Stress in Young Male Handball Players. Molecular and Cellular Biochemistry, 2011, vol. 351, pp. 251–259. DOI: 10.1007/s11010-011-0732-6
7. Fisher-Wellman K., Bloomer R.J. Acute Exercise and Oxidative Stress: a 30 Year History. Dynamic Medicine: DM, 2009, vol. 8, p. 1.
8. Gilbert H.S., Stump D.D., Roth E.F. A Method to Correct for Errors Caused by Generation of Interfering Compounds During Erythrocyte Lipid Peroxidation. Analytical Biochemistry, 1984, vol. 137, pp. 282–286. DOI: 10.1016/0003-2697(84)90086-1
9. Günzler W.A., Vergin H., Müller I., Flohé L. Glutathione Peroxidase VI: the Reaction of Glutahione Peroxidase with Various Hydroperoxides. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie, 1972, vol. 353, pp. 1001–1004.
10. Hanzawa K., Kai M., Hiraga A., Watanabe S. Fragility of Red Cells During Exercise is Affected by Blood pH and Temperature. Equine Veterinary Journal, 1999, suppl. 610–1.
11. Hubner-Wozniak E., Panczenko-Kresowska B., Starczewska-Czapowska J. The Effect of Graded Exercise on Changes in the Activity of Antioxidant Enzymes in Blood of Wrestlers. BiolSport, 1993, vol. 10, pp. 21–22.
12. Iuchi Y. (2012) Anemia Caused by Oxidative Stress. In: Silverberg DS (ed) Anemia. 2012. 480 p. DOI: 10.5772/31404
13. Kaminsky L.A., American College of Sports Medicine., American College of Sports Medicine. ACSM’s Health-Related Physical Fitness Assessment Manual. Wolters Kluwer Health/Lippincott Williams & Wilkins. 2014.
14. Rifkind J.M., Nagababu E., Ramasa- my S., Ravi L.B. Hemoglobin Redox Reactions and Oxidative Stress. Redox Report: Communications in Free Radical Research, 2003, vol. 8, pp. 234–237. DOI: 10.1179/135100003225002817
15. Selby G.B., Eichner E.R. Endurance Swimming, Intravascular Hemolysis, Anemia, and Iron Depletion. New Perspective on Athlete’s Anemia. The American Journal of Medicine, 1986, vol. 81, pp. 791–794. DOI: 10.1016/00029343(86)90347-5
16. Smith J.A., Kolbuch-Braddon M., Gillam I., Telford R.D., Weidemann M.J. Changes in the Susceptibility of Red Blood Cells to Oxidative and Osmotic Stress Following Submaximal Exercise. European Journal of Applied Physiology and Occupational Physiology, 1995, vol. 70, pp. 427–436. DOI: 10.1007/BF00618494
17. Spasic M.B. Antioxidative Defence in Mammals – A Review. Jugoslov Med Biohem, 1993, vol. 12, pp. 1–9.
18. Tauler P., Aguiló A., Guix F., Jiménez F., Villa G., Tur J., Córdova A., Pons A. PreExercise Antioxidant Enzyme Activities Determine the Antioxidant Enzyme Erythrocyte Response to Exercise. J Sports Science, 2005, vol. 23, pp. 5–13. DOI: 10.1080/02640410410001716724
19. Tietze F. Enzymic Method for Quantitative Determination of Nanogram Amounts of Total and Oxidized Glutathione: Applications to Mammalian Blood and Other Tissues. Analytical Biochemistry, 1969, vol. 27, pp. 502–522. DOI: 10.1016/0003-2697(69)90064-5
20. Winterbourn C.C. Haemoglobin Oxidation and Free Radical Production in the Red Cell. Biomedica Biochimica Acta, 1983, vol. 42, pp. 134–138.
21. WMA. WMA Declaration of Helsinki – Ethical Principles for Medical Research Involving Human Subjects. 2013. Available at: http://www.wma.net/en/30publications/10policie s/b3/. Accessed 27.07.2016.
22. Zavodnik L.B., Zavodnik I.B., Niekurzak A., Szosland K., Bryszewska M. Activation of Red Blood Cell Glutathione Peroxidase and Morphological Transformation of Erythrocytes Under the Action of Tert-Butyl Hydroperoxide. Biochemistry and Molecular Biology International, 1998, vol. 44, pp. 577–588. DOI: 10.1080/15216549800201612
References
1. Aebi H. Catalase in Vitro. Methods in Enzymology, 1984, vol. 105, pp. 121–126. DOI: 10.1016/S0076-6879(84)05016-32. Alexandrova A., Eroglu Y., Petrov L., Makaveev R., Georgieva A., Tzvetanova E. Blood Plasma Oxidative Stress Parameters after Maximal Oxygen Consumption Test in Wrestlers. International Journal of Sport Studies, 2016, vol. 6, pp. 359–366.
3. Beauchamp C., Fridovich I. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels. Anal Biochem, 1971, vol. 44, pp. 276–287. DOI: 10.1016/0003-2697(71)90370-8
4. Bogdanska J.J., Korneti P., Todorova B. Erythrocyte Superoxide Dismutase, Glutathione Peroxidase and Catalase Activities in Healthy Male Subjects in Republic of Macedonia. Bratislavské Lekárske Listy, 2003, vol. 104, pp. 108–114.
5. Dane S., Taysi S., Gul M., Akcay F., Gunal A. Acute Exercise Induced Oxidative Stress is Prevented in Erythrocytes of Male Long Distance Athletes. Biology of Sport, 2008, vol. 25, pp. 115–124.
6. Djordjevic D., Cubrilo D., Macura M., Barudzic N., Djuric D., Jakovljevic V. The Influence of Training Status on Oxidative Stress in Young Male Handball Players. Molecular and Cellular Biochemistry, 2011, vol. 351, pp. 251–259. DOI: 10.1007/s11010-011-0732-6
7. Fisher-Wellman K., Bloomer R.J. Acute Exercise and Oxidative Stress: a 30 Year History. Dynamic Medicine: DM, 2009, vol. 8, p. 1.
8. Gilbert H.S., Stump D.D., Roth E.F. A Method to Correct for Errors Caused by Generation of Interfering Compounds During Erythrocyte Lipid Peroxidation. Analytical Biochemistry, 1984, vol. 137, pp. 282–286. DOI: 10.1016/0003-2697(84)90086-1
9. Günzler W.A., Vergin H., Müller I., Flohé L. Glutathione Peroxidase VI: the Reaction of Glutahione Peroxidase with Various Hydroperoxides. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie, 1972, vol. 353, pp. 1001–1004.
10. Hanzawa K., Kai M., Hiraga A., Watanabe S. Fragility of Red Cells During Exercise is Affected by Blood pH and Temperature. Equine Veterinary Journal, 1999, suppl. 610–1.
11. Hubner-Wozniak E., Panczenko-Kresowska B., Starczewska-Czapowska J. The Effect of Graded Exercise on Changes in the Activity of Antioxidant Enzymes in Blood of Wrestlers. BiolSport, 1993, vol. 10, pp. 21–22.
12. Iuchi Y. (2012) Anemia Caused by Oxidative Stress. In: Silverberg DS (ed) Anemia. 2012. 480 p. DOI: 10.5772/31404
13. Kaminsky L.A., American College of Sports Medicine., American College of Sports Medicine. ACSM’s Health-Related Physical Fitness Assessment Manual. Wolters Kluwer Health/Lippincott Williams & Wilkins. 2014.
14. Rifkind J.M., Nagababu E., Ramasa- my S., Ravi L.B. Hemoglobin Redox Reactions and Oxidative Stress. Redox Report: Communications in Free Radical Research, 2003, vol. 8, pp. 234–237. DOI: 10.1179/135100003225002817
15. Selby G.B., Eichner E.R. Endurance Swimming, Intravascular Hemolysis, Anemia, and Iron Depletion. New Perspective on Athlete’s Anemia. The American Journal of Medicine, 1986, vol. 81, pp. 791–794. DOI: 10.1016/00029343(86)90347-5
16. Smith J.A., Kolbuch-Braddon M., Gillam I., Telford R.D., Weidemann M.J. Changes in the Susceptibility of Red Blood Cells to Oxidative and Osmotic Stress Following Submaximal Exercise. European Journal of Applied Physiology and Occupational Physiology, 1995, vol. 70, pp. 427–436. DOI: 10.1007/BF00618494
17. Spasic M.B. Antioxidative Defence in Mammals – A Review. Jugoslov Med Biohem, 1993, vol. 12, pp. 1–9.
18. Tauler P., Aguiló A., Guix F., Jiménez F., Villa G., Tur J., Córdova A., Pons A. PreExercise Antioxidant Enzyme Activities Determine the Antioxidant Enzyme Erythrocyte Response to Exercise. J Sports Science, 2005, vol. 23, pp. 5–13. DOI: 10.1080/02640410410001716724
19. Tietze F. Enzymic Method for Quantitative Determination of Nanogram Amounts of Total and Oxidized Glutathione: Applications to Mammalian Blood and Other Tissues. Analytical Biochemistry, 1969, vol. 27, pp. 502–522. DOI: 10.1016/0003-2697(69)90064-5
20. Winterbourn C.C. Haemoglobin Oxidation and Free Radical Production in the Red Cell. Biomedica Biochimica Acta, 1983, vol. 42, pp. 134–138.
21. WMA. WMA Declaration of Helsinki – Ethical Principles for Medical Research Involving Human Subjects. 2013. Available at: http://www.wma.net/en/30publications/10policie s/b3/. Accessed 27.07.2016.
22. Zavodnik L.B., Zavodnik I.B., Niekurzak A., Szosland K., Bryszewska M. Activation of Red Blood Cell Glutathione Peroxidase and Morphological Transformation of Erythrocytes Under the Action of Tert-Butyl Hydroperoxide. Biochemistry and Molecular Biology International, 1998, vol. 44, pp. 577–588. DOI: 10.1080/15216549800201612
Опубликован
2019-04-29
Как цитировать
Александрова, А., Петров, Л., Макавеев, Р., Цветанова, Э., Георгиева, А., & Колимечков, С. (2019). ОКСИДАТИВНЫЙ СТАТУС ЭРИТРОЦИТОВ ПОСЛЕ МАКСИМАЛЬНОГО АЭРОБНОГО ТЕСТА У БОРЦОВ. Человек. Спорт. Медицина, 19(1), 15-21. https://doi.org/10.14529/hsm190102
Выпуск
Раздел
Физиология
Copyright (c) 2019 Человек. Спорт. Медицина
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