GLUCOCORTICOID-RELATED CHANGE OF BEHAVIORAL ACTIVITY AND FREE-RADICAL OXIDATION IN THE BRAIN AT ADMINISTRATION OF EXOGENOUS GLUCOCORTICOID

Dodokhova M.

doi:10.14529/hsm160115

M. Dodokhova Rostov-on-Don State Medical University, Rostov-on-Don, Russian Federation dodohova@mail.ru

DOI: https://doi.org/10.14529/hsm160115

Abstract

Aim. To study how exogenous glucocorticoid drug triamcinolone acetonide influences the correlation between behavioral activity and levels in the brain. Materials and methods. To assess the glucocorticoid-related changes of free-radical oxidation the animals were administered glucocorticosteroid drug triamcinolone acetonide. Animals’ behavioral reactions were studied using open-field actograph, and the levels of lipid peroxidation products and carbonylated proteins in the brain were measured. Results. Administration of exogenous glucocorticoid was associated with decrease in orientation response (number of upright postures) and self-cleaning behavior (grooming) when animals were tested in the open-field actograph. The exogenous glucocorticosteroid significantly increased the levels of lipid peroxidation and decreased protein carbonylation in the brain with parallel decrease of levels of MAO-B activity. Conclusion. The obtained results indicate that the exogenous glucocorticoid due to induction of microsomal enzymes of the tissue glucocorticoid metabolism leads to decrease in the levels of corticosterone. With this background, the level of MAO-B activity is decreased, and the level of lipid peroxidation is increased. The observed neurochemical changes specify the behavioral disorders.

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