DYNAMICS OF PATHOMORPHOLOGICAL CHANGES IN RAT ISCHEMIC SPINAL CORD AFTER TREATMENT WITH RECOMBINANT ERYTHROPOIETIN (EXPERIMENTAL STUDY)
Abstract
Aim is to study the dynamics of morphological changes in spinal cord ischemic lesion in rats influenced by recombinant erythropoietin (REP). Materials and methods. The study was conducted on 40 mature rats. The animals were divided into two series of experiment 20 animals each. The first series of animals was the group of comparison with spinal cord ischemia model. In the second series the animals were administered intraperitoneally with 1000 international units of REP in 3, 24, and 48 hours. After the animals had been sacrificed, the spinal cord was removed for further histological and morphometric study. The obtained results were processed using analysis of variance. The statistical significance of differences between compared parameters of the groups was assessed with the Mann-Whitney U test. The differences were considered significant at p &< 0.05 which corresponds to 95 % confidence interval. Results. The study of the spinal cord specimen showed that REP administration results in significantly higher levels of normal neurons and blood vessels, and in significantly lower count of chromatolytic neurons and ghost cells at all stages of experiment. Conclusion. At the early stages of experiment the REP effects increases ischemic tolerance of neurons, enhances the proliferation rate of gliocytes and endotheliocytes with development of a new blood stream.References
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3. Celik M., Gokmen N., Erbayraktar S. Erythropoietin Prevents Motor Neuron Apoptosis and Neurologic Disability in Experimental Spinal Cord Ischemic Injury. Proc Natl Acad Sci USA. 2002, vol. 99, pp. 2258–2263. DOI: 10.1073/pnas.042693799
4. Fisher M., Takano K. Ballierie's Clinical Neurology, Cerebrovascular Disease. London, 1995, pp. 279–296.
5. Inagaki S., Kitos S. Peptides in the Peripheral Nervous System. Progr. Brain Res. 1986, vol. 66, pp. 269–316. DOI: 10.1016/S0079-6123(08)64607-5
6. Masuda S., Nagan M., Takahata K. Functional Erythropoietin Receptor of the Cells with Keural Characteristies. Comparison with Receptor Properties of Erythroid Cells. J. Biol. Chem. 1993, vol. 268, pp. 11208–11216.
7. Santhanam A.V., Katusic Z.S. Erythropoietin and Cerebral Vascular Protection. Role of Nitric Oxide. Acta Pharmacol Sin. 2006, vol. 27, pp. 1389–1394. DOI: 10.1111/j.1745-7254.2006.00441.x
8. Siren A.L., Fratelli M., Brines M. Erythropoietin Prevent Neuronal Apoptosis After Cerebral Ischemia and Metabolic Stress. Proc Natl Acad Sci USA. 2001, vol. 98, pp. 4044–4049. DOI: 10.1073/pnas.051606598
9. Symon L., Branston N.M., Strong A.J. The Concepts of Thresholds of Ischaemia in Relation to Brain Structure and Function. J Clin Pathol Suppl (R Coll Pathol). 1977, vol. 11, pp. 149–154. DOI: 10.1136/jcp.s3-11.1.149
10. Wang L., Zhang Z., Wang Y., Zhang R., Chopp M. Treatment of Stroke with Erythropoietin Enhances Neurogenesis and Angiogenesis and Improves Neurological Function in Rats. Stroke. 2004, vol. 35, pp. 1732–1737. DOI: 10.1161/01.STR.0000132196.49028.a4
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Published
2016-02-01
How to Cite
Volodchenko, A., Giniatullin, R., Kosel, A., & Astakhova, L. (2016). DYNAMICS OF PATHOMORPHOLOGICAL CHANGES IN RAT ISCHEMIC SPINAL CORD AFTER TREATMENT WITH RECOMBINANT ERYTHROPOIETIN (EXPERIMENTAL STUDY). Human. Sport. Medicine, 16(1), 51-55. https://doi.org/10.14529/hsm160108
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Clinical and Experimental Medicine
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