INFLUENCE OF BESTIM IMMUNOMODULATOR ON IMMUNE STATUS AND FREE-RADICAL OXIDATION IN PATIENTS WITH CHRONIC PYELONEPHRITIS ASSOCIATED WITH UROLITHIASIS
Keywords:
pyelonephritis, urolithiasis, immune status, free-radical oxidation, Bestim
Abstract
Aim of research is to study the influence of Bestim immunomodulator on indices of immune status and free-radical oxidation in patients with chronic calculous pyelonephritis depending on the phase of inflammation. Introduction. Changes in immune status and active processes of free-radical oxidation in patients with chronic secondary pyelonephritis (CSP) associated with urolithiasis (UL) are significant in the pathogenesis of underlying disease and its complications. Therefore, it is reasonable to supplement complex therapy with antioxidant and immunomodulatory agents, particularly with Bestim (?–D–glutamyl–L–tryptophan sodium salt). Materials and Methods. We conducted short-term prospective randomized study of effectiveness of Bestim immunomodulatory agent application in patients with chronic secondary pyelonephritis associated with UL as a part of standard conservative therapy combined with surgical treatment. In accordance with inclusion and exclusion criteria we selected 164 patients with chronic secondary pyelonephritis associated with UL who were under medical treatment in the department of urology in the Road Clinical Hospital, Chelyabinsk, and in the department of urology in the Regional Clinical Hospital, Chelyabinsk. Results. It has been established that in patients with CSP associated with UL neutrophil leukocytosis is developed, absorbing lysosomal activity is enhanced, NBT-reductive capacity of neutrophils is decreased, concentration of IL-1?, IL-4, IL-8, and IFN-? is increased, and activity of superoxide dismutase (SOD) b catalase is decreased. More expressed changes in immune status are observed in patients with UL-associated CSP in the active phase of inflammation. After standard combined therapy patients with active phase of CSP associated with UL have lower count of band neutrophils in blood; absorbing lysosomal activity is still enhanced, NBT-reductive capacity of neutrophils is decreased, concentration of IL-1?, IL-4, IL-8, and IFN-? is increased, and activity of superoxide dismutase and catalase in plasma and erythrocytes is decreased. Conclusion. If the combined therapy in patients with CSP associated with UL is supplemented with Bestim the changes observed during the active phase of inflammation become more expressed: the count of band neutrophils in blood is decreased, absorbing activity is restored, NBT-reductive capacity of neutrophils keeps on decreasing, concentration of IL-1?, IL-8, and IFN-? is decreased, and concentration of IL-2 and IL-4 in blood is increased.References
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2. Bychkovskikh V.A. [The Cytokine Status of Patients with Chronic Pyelonephritis Only Kidney in the Early Postoperative Period, when Used in the Complex Therapy of Immunomodulator Bestim]. Tsitokiny i vospalenie [Cytokines and Inflammation], 2011, vol. 10, no. 1, pp. 60–63. (in Russ.)
3. Bychkovskikh V.A., Dolgushin I.I., Bordunovskiy V.N., Kukarkin N.Yu. [Influence Bestim on Clinical and Immunological Characteristics of Patients with Chronic Pyelonephritis in the Early Postoperative Period]. Bulletin of South Ural State University. Ser. Education, Healthcare Service, Physical Education, 2009, no. 20 (153), pp. 51–57. (in Russ.)
4. Osikov M.V., Grigor'ev T.A., Fedosov A.A. [Effect on Platelet Erythropoietin Functional Activity]. Sovremennye problemy nauki i obrazovaniya [Modern Problems of Science and Education], 2012, no. 6. Available at: http://www.science-education.ru/ru/article/view?id=7450 (accessed 16.05.2016).
5. Mayanskiy A.N., Viksman M.K. Sposoby otsenki funktsional'noy aktivnosti neytrofilov cheloveka po reaktsii vosstanovleniya nitrosinego tetrazoliya: metodicheskie rekomendatsii [Methods for Evaluating the Functional Activity of Human Neutrophils by the Reduction Reaction of Nitro Blue Tetrazolium. Method. Rivers.]. Kazan', 1979. 11 p.
6. Koralyuk M.A., Ivanova L.I., Mayorova I.G., Tokarev V.E. [The Method for Determining the Activity of Catalase]. Laboratornoe delo [Laboratory Business], 1988, no. 1, pp. 16–19. (in Russ.)
7. Osikov M.V., Krivokhizhina L.V., Mal'tsev A.V. [Analysis Efferent Properties Ceruloplasmin and Alpha-1-Acid Glycoprotein in Experimental Peritonitis]. Efferentnaya terapiya [Maltsev Efferent Therapy], 2006, vol. 12, no. 4, pp. 36–39. (in Russ.)
8. Osikov M.V. [Orosomucoid Role in the Regulation of Plasma Proteolytic Systems in Experimental Renal Failure]. Byulleten' eksperimental'noy biologii i meditsiny [Bulletin of Experimental Biology and Medicine], 2009, vol. 148, no. 7, pp. 27–30. (in Russ.)
9. Osikov M.V., Akhmatov K.V., Fedosov A.A. [On the Mechanism of the Effect of Erythropoietin on the Affective Status in Patients with Chronic Renal Failure on Hemodialysis]. Fundamental'nye issledovaniya [Basic Research], 2012, no. 7 (1), pp. 140–145. (in Russ.)
10. Osikov M.V., Akhmatov K.V., Krivokhizhina L.V. [The Pathophysiological Analysis of the Effect of Erythropoietin on the Psychological Status of Patients with Chronic Renal Failure on Hemodialysis]. Bulletin of South Ural State University. Ser. Education, Healthcare Service, Physical Education, 2010, no. 19 (195), pp. 110–116. (in Russ.)
11. Osikov M.V. Reaktivnye izmeneniya kletochno-gumoral'noy sistemy organizma kak tipovoy patologicheskiy protsessii ego regulyatsiya reaktantami ostroy fazy. Avtoref. dokt. diss. [Reactive Changes in Cell-Humoral Systems of the Body As a Typical Pathological Process of Its Regulation of the Acute Phase Reactants. Abstract of doct. diss.]. Chelyabinsk, 2008. 44 p.
12. Osikov M.V., Grigor'ev T.A. [Effect of Erythropoietin on Activity of Plasma Proteolytic Systems in Experimental Renal Failure]. Byulleten' eksperimental'noy biologii i meditsiny [Bulletin of Experimental Biology and Medicine], 2012, vol. 153, no. 1, pp. 27–30. (in Russ.)
13. Osikov M.V., Grigor'ev T.A. [The Role of Erythropoietin in the Correction of Vascular-Platelet Hemostasis in Patients with End-Stage Chronic Renal Failure]. Fundamental'nye issledovaniya [Basic Research], 2011, no. 9 (3), pp. 462–466. (in Russ.)
14. Osikov M.V., Grigor'ev T.A., Fedosov A.A. [The Role of Erythropoietin in the Implementation of Platelet–Cell Interactions in the Blood in Patients with Chronic Renal Failure]. Fundamental'nye issledovaniya [Basic Research], 2012, no. 10 (2), pp. 285–289. (in Russ.)
15. Freydlin I.S. Metody izucheniya fagotsitiruyushchikh kletok pri otsenke immunnogo statusa cheloveka [Methods of Study of Phagocytic Cells in the Evaluation of Human Immune Status]. Leningrad, 1986. 37 p.
16. Chevari S., Chaba I., Sekey I. [The Role of Superoxide Dismutase in Oxidative Processes Cells and the Method of its Determination in Biological Materials]. Laboratornoe delo [Laboratory Case], 1985, no. 11, pp. 678–681. (in Russ.)
17. Volchegorskiy I.A., Dolgushin I.I., Kolesnikov O.L. Eksperimental'noe modelirovanie i laboratornaya otsenka adaptivnykh reaktsiy organizma [Experimental Modeling and Laboratory Evaluation of Adaptive Reactions of the Organism]. Chelyabinsk, 2000. 167 p.
18. Khan S.R. Reactive Oxygen Species, Inflammation and Calcium Oxalate Nephrolithiasis. Transl. Androl. Urol., 2014, vol. 3 (3), pp. 256–276.
19. Han H., Segal A.M., Seifter J.L., Dwyer J.T. Nutritional Management of Kidney Stones (Nephrolithiasis). Clin. Nutr. Res., 2015, vol. 4 (3), pp. 137–152.
20. Tang X., Lieske J.C. Acute and Chronic Kidney Injury in Nephrolithiasis. Curr. Opin. Nephrol. Hypertens., 2014, vol. 23 (4), pp. 385–390.
21. Spencer J.D., Schwaderer A.L., Becknell B. The Innate Immune Response During Urinary Tract Infection and Pyelonephritis. Pediatr. Nephrol., 2014, vol. 29 (7), pp. 1139–1149.
References on translit
Published
2016-06-01
How to Cite
Telesheva, L., Kazimirchuk, A., Bychkovskikh, V., & Osikov, M. (2016). INFLUENCE OF BESTIM IMMUNOMODULATOR ON IMMUNE STATUS AND FREE-RADICAL OXIDATION IN PATIENTS WITH CHRONIC PYELONEPHRITIS ASSOCIATED WITH UROLITHIASIS. Human. Sport. Medicine, 16(2), 5-17. https://doi.org/10.14529/hsm160201
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Clinical and Experimental Medicine
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