BIOSAFE GRAIN-BASED DRINKS FOR SPORTS NUTRACEUTICALS

Potoroko I.; Ruskina А.; Malinin А.; Nenashev А.

doi:10.14529/hsm250315

I. Potoroko South Ural State University, Chelyabinsk, Russia https://orcid.org/0000-0002-3059-8061 potorokoii@susu.ru
А. Ruskina South Ural State University, Chelyabinsk, Russia https://orcid.org/0000-0002-2451-9339 ruskinaaa@susu.ru
А. Malinin South Ural State University, Chelyabinsk, Russia https://orcid.org/0000-0001-9270-5945 malininav@susu.ru
А. Nenashev South Ural State University, Chelyabinsk, Russia https://orcid.org/0000-0002-6881-8963 genri50374@mail.ru

DOI: https://doi.org/10.14529/hsm250315

Keywords: sports nutraceuticals, biosafety, grain-based drinks, non-thermal disinfection, added value

Abstract

Abstract. Aim. This study aimed to develop a biosafe grain-based drink for sports nutraceutical applications, providing enhanced nutritional value. Materials and methods. The multimethod approach incorporated ground-state quantum chemical calculations (Hartree-Fock method, HF); in silico molecular dynamics simulations of binding; Fourier transform infrared spectroscopy for functional group characterization; DPPH assay for antioxidant activity analysis (AOA) (%); bioactivity index (BAI, %) through in vitro gastrointestinal modeling; and biosafety assessment using Paramecium caudatum toxicity assay. Result. The optimized grain-based drink exhibited antioxidant activity (43–57%, DPPH) and bioactivity (69.82–74.24% BAI) across all model samples, supporting its potential application as a functional nutraceutical product. Regarding biosafety, the migration risks of Fusarium spp. mycotoxins (MT), particularly Zearalenone (ZEN) and its derivative zeranol, were identified. These metabolites form stable bonds with grain matrix proteins, ensuring product safety. Conclusion. The methodology aligns with import-substitution strategies and agro-industrial by-product valorization.

Author Biographies

I. Potoroko , South Ural State University, Chelyabinsk, Russia

Doctor of Technical Sciences, Professor, Head of the Department of Food and Biotechnology, South Ural State University, Chelyabinsk, Russia.

А. Ruskina , South Ural State University, Chelyabinsk, Russia

Candidate of Technical Sciences, Associate Professor of the Department of Food and Biotechnology, South Ural State University, Chelyabinsk, Russia.

А. Malinin , South Ural State University, Chelyabinsk, Russia

Assistant, Department of Food and Biotechnology, South Ural State University, Chelyabinsk, Russia.

А. Nenashev , South Ural State University, Chelyabinsk, Russia

Student of the Department of Theory and Methods of Physical Culture and Sports, South Ural State University, Chelyabinsk, Russia.

References

References on translit

BIOSAFE GRAIN-BASED DRINKS FOR SPORTS NUTRACEUTICALS

Abstract

Author Biographies

References

Most read articles by the same author(s)