PHYSIOLOGICAL MECHANISMS OF THE GENETIC REGULATION OF FUNCTIONAL PERFORMANCE AND ENDURANCE IN COMBAT ATHLETES DURING ADAPTATION TO PHYSICAL LOAD
Keywords:
Adaptation, combat athletes, genes, alleles, efficiency
Abstract
Aim. The article deals with studying physiological mechanisms of the polymorphic variants of genes H1F1A Pro/Ser, influencing adaptation in combat athletes during physical load. Materials and methods. We studied combat athletes of various ranks from a sports school in Chelyabinsk: athletes of 1st, 2nd, and 3rd ranks (youth ranking) – 23 persons (control group); athletes of 1st, 2nd, and 3rd ranks (adult ranking) – 27 persons (experimental group 1); professional athletes (Candidate for Master of Sport and Master of Sport) – 26 persons (experimental group 2). We studied polymorphic variants of genes, regulating the cardiovascular system, performance, and endurance in combat athletes (HIF1A Pro/Ser). We performed the amplification of the fragments of genomic DNA, containing polymorphic parts, by polymerase chain reaction (PCR) in iQ5 Bio-Rad (the USA) and GeneAmp PCR System 9700 (Applied Biosystems, the USA) amplifiers. Primers were synthesized by Evrogen company (Russia). Results. During the typing of Pro582Ser polymorphism of H1F1A gene, we revealed three genotypes and two alleles. Only in the experimental group 2, H1F1A *Ser/Ser genotype has a frequency of 76.47%; for the same sample, the frequency of H1F1*Ser allele is 87.2% and of H1F1A*Pro allele is 12.75%. Conclusion. We performed the analysis of the polymorphic loci of molecular genetic markers (HIF1A Ser/Ser, HIF1A*Ser). This allowed us to determine the cardiorespiratory and aerobic glycolytic system performance in wrestlers during their adaptation to physical load. Our analysis revealed that there is a direct correlation between the high level of integral functional preparedness in combat athletes and athletes’ gene alleles (HIF1A Ser/Ser, HIF1A*Ser). To achieve success in combat sports, the optimal number of these alleles is from 4 to 6.References
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2. Saraykin D.A., Bacherikov E.L., Kamskova Yu.G., Pavlova V.I. [Study of Physiological Indices of Taekwondokas in Case of Sensory Conflict]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2017, no. 12, pp. 62–64. (in Russ.)
3. Meerson F.Z. Adaptacionnaya medicina: koncepciya dolgovremennoj adaptacii [Adaptation Medicine. Concept of Long-Term Adaptation]. Moscow, Deal Publ., 1993. 138 p.
4. Terzi M.S., Lekontsev E.V., Saraykin D.A., Pavlov V.I., Kamskova Yu.G. [Molecular Genetic Determination of Functional Performance of Combatants of Different Skill Levels]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2016, no. 7, pp. 21–24. (in Russ.)
5. Rogozkin V.A. [Interpretation of Human Genome and Sport]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2001, no. 6, pp. 60–63. (in Russ.)
6. Sergienko L.P. Osnovy sportivnoj genetiki [Fundamentals of Sports Genetics]. Kiev, High School Publ., 2004. 631 p.
7. Feropontov M.A., Lekontsev E.V. [Research of Polymorphic Options of the Genes Affecting Sports Success in High-Speed Strength Sports]. Materialy XXII regional'naya nauchno-metodicheskaya konferenciya s mezhdunarodnym uchastiem “Optimizaciya uchebno-vospitatel'nogo processa v obrazovatel'nyh uchrezhdeniyah fizicheskoj kul'tury” [Materials XXII a Regional Scientific and Methodical Conference with the International Participation. Optimization of Teaching and Educational Process in Educational Institutions of Physical Culture], 2012, pp. 212–214. (in Russ.)
8. Golberg N.D., Astratenkova I.V., Akhmetov I.I., Rogozkin V.A. [Epigenetic Modifications During Exercise]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2018, no. 2, pp. 51–53. (in Russ.)
9. Isaev A.P., Erlikh V.V., Bakhareva A.S., Saraykin D.A., Pavlova V.I., Maleev D.O. Effects of Short- and Long-Term Adaptation to the Middle-Altitude Hypoxia on the Condition of Athletes Practicing Cyclic and Acyclic Sports. Minerva Ortopedica e Traumatologica, 2018, vol. 69, suppl. 1, no. 3, pp. 31–42.
10. Saraykin D.A. Genetic Prerequisites of Sports Success of Sportsmen Going in for Combat Sports. Journal of Pharmaceutical Sciences and Research, 2017, vol. 9 (9), pp. 1569–1572.
11. Pescatello L.S., Devaney J.M., Hubal M.J., Thompson P.D., Hoffman E.P. Highlights from the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength or FAMuSS Study. Biomed Res. Int., 2013, pp. 43–57. DOI: 10.1155/2013/643575
12. Prior S.J., Hagberg J.M., Phares D.A., Brown M.D., Fairfull L., Fen-ell R.E., Roth S.M. Sequence Variation in Hypoxia-Inducible Factor 1 Alpha (HIF1A): Association with Maximal Oxygen Consumption. Physiol. Genomics, 2003, vol. 15, pp. 20–26. DOI: 10.1152/physiolgenomics.00061.2003.
2. Saraykin D.A., Bacherikov E.L., Kamskova Yu.G., Pavlova V.I. [Study of Physiological Indices of Taekwondokas in Case of Sensory Conflict]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2017, no. 12, pp. 62–64. (in Russ.)
3. Meerson F.Z. Adaptacionnaya medicina: koncepciya dolgovremennoj adaptacii [Adaptation Medicine. Concept of Long-Term Adaptation]. Moscow, Deal Publ., 1993. 138 p.
4. Terzi M.S., Lekontsev E.V., Saraykin D.A., Pavlov V.I., Kamskova Yu.G. [Molecular Genetic Determination of Functional Performance of Combatants of Different Skill Levels]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2016, no. 7, pp. 21–24. (in Russ.)
5. Rogozkin V.A. [Interpretation of Human Genome and Sport]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2001, no. 6, pp. 60–63. (in Russ.)
6. Sergienko L.P. Osnovy sportivnoj genetiki [Fundamentals of Sports Genetics]. Kiev, High School Publ., 2004. 631 p.
7. Feropontov M.A., Lekontsev E.V. [Research of Polymorphic Options of the Genes Affecting Sports Success in High-Speed Strength Sports]. Materialy XXII regional'naya nauchno-metodicheskaya konferenciya s mezhdunarodnym uchastiem “Optimizaciya uchebno-vospitatel'nogo processa v obrazovatel'nyh uchrezhdeniyah fizicheskoj kul'tury” [Materials XXII a Regional Scientific and Methodical Conference with the International Participation. Optimization of Teaching and Educational Process in Educational Institutions of Physical Culture], 2012, pp. 212–214. (in Russ.)
8. Golberg N.D., Astratenkova I.V., Akhmetov I.I., Rogozkin V.A. [Epigenetic Modifications During Exercise]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2018, no. 2, pp. 51–53. (in Russ.)
9. Isaev A.P., Erlikh V.V., Bakhareva A.S., Saraykin D.A., Pavlova V.I., Maleev D.O. Effects of Short- and Long-Term Adaptation to the Middle-Altitude Hypoxia on the Condition of Athletes Practicing Cyclic and Acyclic Sports. Minerva Ortopedica e Traumatologica, 2018, vol. 69, suppl. 1, no. 3, pp. 31–42.
10. Saraykin D.A. Genetic Prerequisites of Sports Success of Sportsmen Going in for Combat Sports. Journal of Pharmaceutical Sciences and Research, 2017, vol. 9 (9), pp. 1569–1572.
11. Pescatello L.S., Devaney J.M., Hubal M.J., Thompson P.D., Hoffman E.P. Highlights from the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength or FAMuSS Study. Biomed Res. Int., 2013, pp. 43–57. DOI: 10.1155/2013/643575
12. Prior S.J., Hagberg J.M., Phares D.A., Brown M.D., Fairfull L., Fen-ell R.E., Roth S.M. Sequence Variation in Hypoxia-Inducible Factor 1 Alpha (HIF1A): Association with Maximal Oxygen Consumption. Physiol. Genomics, 2003, vol. 15, pp. 20–26. DOI: 10.1152/physiolgenomics.00061.2003.
References on translit
1. Isaev A.P., Ehrlich V.V., Temnikova I.V., Epishev V.V. [Interrelations Between Genetic Markers and Physical Fitness of Athletes of Strength Sports]. Mater. Mezhdunar. nauch.-metod. konf.: Nauchnometodicheskoe obespechenie i soprovozhdenie sistemy fizicheskogo vospitaniya i sportivnoj podgotovki v kontekste vnedreniya kompleksa GTO [Materials of the International Scientific and Methodical Conference: Scientific and Methodical Ensuring and Maintenance of a System of Physical Training and Sports Preparation in the Context of Introduction of the GTO Complex], 2015, pp. 41–45. (in Russ.)2. Saraykin D.A., Bacherikov E.L., Kamskova Yu.G., Pavlova V.I. [Study of Physiological Indices of Taekwondokas in Case of Sensory Conflict]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2017, no. 12, pp. 62–64. (in Russ.)
3. Meerson F.Z. Adaptacionnaya medicina: koncepciya dolgovremennoj adaptacii [Adaptation Medicine. Concept of Long-Term Adaptation]. Moscow, Deal Publ., 1993. 138 p.
4. Terzi M.S., Lekontsev E.V., Saraykin D.A., Pavlov V.I., Kamskova Yu.G. [Molecular Genetic Determination of Functional Performance of Combatants of Different Skill Levels]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2016, no. 7, pp. 21–24. (in Russ.)
5. Rogozkin V.A. [Interpretation of Human Genome and Sport]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2001, no. 6, pp. 60–63. (in Russ.)
6. Sergienko L.P. Osnovy sportivnoj genetiki [Fundamentals of Sports Genetics]. Kiev, High School Publ., 2004. 631 p.
7. Feropontov M.A., Lekontsev E.V. [Research of Polymorphic Options of the Genes Affecting Sports Success in High-Speed Strength Sports]. Materialy XXII regional'naya nauchno-metodicheskaya konferenciya s mezhdunarodnym uchastiem “Optimizaciya uchebno-vospitatel'nogo processa v obrazovatel'nyh uchrezhdeniyah fizicheskoj kul'tury” [Materials XXII a Regional Scientific and Methodical Conference with the International Participation. Optimization of Teaching and Educational Process in Educational Institutions of Physical Culture], 2012, pp. 212–214. (in Russ.)
8. Golberg N.D., Astratenkova I.V., Akhmetov I.I., Rogozkin V.A. [Epigenetic Modifications During Exercise]. Teoriya i praktika fizicheskoy kul’tury [Theory and Practice of Physical Culture], 2018, no. 2, pp. 51–53. (in Russ.)
9. Isaev A.P., Erlikh V.V., Bakhareva A.S., Saraykin D.A., Pavlova V.I., Maleev D.O. Effects of Short- and Long-Term Adaptation to the Middle-Altitude Hypoxia on the Condition of Athletes Practicing Cyclic and Acyclic Sports. Minerva Ortopedica e Traumatologica, 2018, vol. 69, suppl. 1, no. 3, pp. 31–42.
10. Saraykin D.A. Genetic Prerequisites of Sports Success of Sportsmen Going in for Combat Sports. Journal of Pharmaceutical Sciences and Research, 2017, vol. 9 (9), pp. 1569–1572.
11. Pescatello L.S., Devaney J.M., Hubal M.J., Thompson P.D., Hoffman E.P. Highlights from the Functional Single Nucleotide Polymorphisms Associated with Human Muscle Size and Strength or FAMuSS Study. Biomed Res. Int., 2013, pp. 43–57. DOI: 10.1155/2013/643575
12. Prior S.J., Hagberg J.M., Phares D.A., Brown M.D., Fairfull L., Fen-ell R.E., Roth S.M. Sequence Variation in Hypoxia-Inducible Factor 1 Alpha (HIF1A): Association with Maximal Oxygen Consumption. Physiol. Genomics, 2003, vol. 15, pp. 20–26. DOI: 10.1152/physiolgenomics.00061.2003.
Published
2018-11-01
How to Cite
Saraykin, D. (2018). PHYSIOLOGICAL MECHANISMS OF THE GENETIC REGULATION OF FUNCTIONAL PERFORMANCE AND ENDURANCE IN COMBAT ATHLETES DURING ADAPTATION TO PHYSICAL LOAD. Human. Sport. Medicine, 18(S), 27-32. https://doi.org/10.14529/hsm18s04
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Physiology
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